(Biology) CD44 and Foxp3 antibodies and their use in T-cell studies

August 30th, 2010 Admin Posted in Your source for sciene news and information |

By James Dacanay

A number of antibodies are specific to CD44. A transmembrane cell-surface glycoprotein, it is expressed on a number of cell types including B cells, macrophages, monocytes, thymocytes and peripheral T cells. Its main function is in cell adhesion, although it is also involved in cell-to-cell interactions and cell migrations.

CD44 features in a number of cellular processes, including hematopoiesis, lymphocyte homing and leukocyte activation. It is also expressed in the metastasis and development of certain tumours. Antibody suppliers, such as us at Novus Biologicals, provide CD44 reagents for cell surface marker studies, as CD44 is expressed in some breast and prostate cancer stem cell lines.

Foxp3 is a highly conserved transcriptional regulator of the forkhead/winged-helix protein family, and is essential for homeostasis in the immune system. A marker protein, it is specifically expressed at high concentrations in CD4+CD25+ regulatory T cells, where it has a regulatory role and can help suppress T cell proliferation. Although CD44 is known to be expressed in regulatory T-cells, its role is less clear. However, in 2009 Liu et al demonstrated a link between Foxp3 and CD44 expression.

The research involved analysis of Foxp3 levels in CD44 positive T regulatory (Treg) cells, using a variety of assay techniques. IL10 antibodies were also used to determine levels of the immunoregulatory cytokine interleukin 10 in a variety of thymocyte subsets. Suppressor activity in populations of spleen Treg cells was also investigated.

The results showed two distinct subsets of Treg cells in the thymus, each with specific staining features. Multi-assay studies at cellular and molecular levels indicated a positive correlation between CD44 and Foxp3 expression in thymocytes. Additionally, it was shown the suppressive effect of Treg cells on T cell production could be blocked by anti-IL10 antibodies. The study showed that CD44 has functional relevance in determining Treg subsets, which are modulated by IL10 and Foxp3. We have recently added new CD44 products to our antibody database, and look forward to seeing further research done in this area.

The Article is written by novusbio.com/ providing antibodies and antibody database Services. Visit http://www.novusbio.com/ for more information on novusbio.com/Products & Services

The Difference between Contract and College/University Science

By Jordon Casinger

Have you ever thought about the reasons why people of science go in for research? Of course, these reasons may be absolutely different. So let us enumerate some of them.

Contract science workers

Some scientists work in this or that department on contract basis. The scientist works there to do a certain type of research. In other words such scientists work there because they have signed the contract and they are obliged to work for the company/college/university till termination of the contract. Of course, the contract may be prolonged. But it depends on many circumstances. But by the end of the contract many scientists feel frustrated. This happens because contracts are usually short and there is not enough time to manage everything that was planned. There is no desire to work for civilization.

The Advantage of College/University Researchers

Other workers of science work as lecturers or tutors. They work not only with students but they also are to do a certain research. But this research is of absolutely different nature. Contract researchers are to do the research appointed by a company, but scientists who work at college/university can choose the sphere and the topic of the research. The second type of research gives scientists freedom of choice. And this is very important as research workers are also creative people as well as painters.

Moreover, scientists - both those working on contract basis and college/university scientists - enjoy their work because it gives them a chance to be engaged in the favourite occupation. They merge into the scientific atmosphere where all the staff uses terms in their speech. Besides it gives the opportunity to develop scientific skills and to exchange opinions about this or that scientific sphere. Nobody would disagree that it is rather pleasant to find something new and to make this something new work for the benefit of society.

Jordon Casinger works in academic paper writing services as a professional academic writer. Pursuing her own ambitious scientific career, she helps countless students worldwide with professional custom assignments and custom essays writing.

Scientific Careers: The Best Professions for Postgraduates

By Randy Wheeler

In case you are searching for an occupation connected with science, then you can be presented with the list of the most interesting scientific careers. The professions provided on the list can be attractive both to those who plan to choose a traditional career path and for those who are looking for alternative and unconventional ways for promotion.

The list of the best scientific careers:

1.Sport science

This field of knowledge includes the exploration of all equipment and technology applied in sport, strategies and tactics worked out by coaches, and facilities aimed at improving the conditions for sportsmen.

2.Computer science

People engaged this profession deal with software and hardware, mobile equipment, and informational exchange. Computer professionals work on devising different methods of human-computer interaction. This profession is rather challenging and difficult at the same time.

3.Health science

This field of science explores different techniques in medical care. It also studies different diseases and investigates their possible treatments.

4.Social science

Social studies are connected with the analysis of process taking place within our society. It considers the nature of conflicts and the role of communication in human interaction.

5.Aquatic science

Water is life and, therefore, scholars are involved in the study of marine life and different processes connected with them.

The list of the most spread scientific career:

1.Math and science

This profession is rather complicated, as it requires the knowledge of different formulas and algebraic equations. Nevertheless, this science is indispensible to other sciences.

2.Library science

This profession is destined for those who love books and everything connected with them.

3.Biological science

Flora and fauna of our planet has much in store for investigation. Therefore, the study of nature origin and evolution is an intricate and ongoing process.

4.Information science

Mass media, breaking news, journalism all these issues are included within the framework of informational science.

Randy Wheeler is a professional academic writer in online writing service. Having degrees in history and sociology, he assists students from all over the world with writing an academic paper and essay writing online.

Your Source For Sciene News And Information